| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192459 |
| Chromosome: | chromosome 13 |
| Location: | 2558950 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580850 | PRPL22 | Chloroplast ribosomal protein L22/L18; (1 of 1) PTHR13501//PTHR13501:SF3 - CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAACGTGACGATGCGGTGCCTGGTACT |
| Internal bar code: | TGTCGACTGACTTTCTTGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.6777 |
| LEAP-Seq n confirming: | 5566 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGTGTGGTGGAGGAAAG |
| Suggested primer 2: | CTATTGCGAGATTTGCGACA |