| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192491 |
| Chromosome: | chromosome 7 |
| Location: | 4263952 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGCGGTCATGGCGGTCATGGATGAAG |
| Internal bar code: | CGTAGGAAATTCTCTCTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.311 |
| LEAP-Seq n confirming: | 1009 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGAGGTCTCTTTCGCAC |
| Suggested primer 2: | AACCATGACAGTAGGCGGAC |