Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.192491 |
Chromosome: | chromosome_7 |
Location: | 4264291 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g341750 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACCAGCACCGCGCTCCGTAGCGGCGGCCT |
Internal bar code: | TACCATCGCGGACGATCGGATCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 616 |
LEAP-Seq percent confirming: | 99.26 |
LEAP-Seq n confirming: | 6707 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAGCACTACTTCGGACAGC |
Suggested primer 2: | CTTCCTCTAGTGGTGGCTGC |