Insertion junction: LMJ.RY0402.192531_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.192531
Chromosome:chromosome 11
Location:1911040
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g468200 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTACCTGGAACACACGCCACGCTACGCT
Internal bar code:TTAACTAGTCGCAAATTTACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:503
LEAP-Seq percent confirming:99.779
LEAP-Seq n confirming:903
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TACGCACATCCATGACACCT
Suggested primer 2:AAACGCAAACCTTATCACCG