| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192541 |
| Chromosome: | chromosome 10 |
| Location: | 755336 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422850 | (1 of 5) PTHR11614:SF87 - MONOGLYCERIDE LIPASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCAAGGCTTGGCAAGGCGCACATGAG |
| Internal bar code: | ACCTCGATGGGGTACACTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATTGTTTGGGTTTTGA |
| Suggested primer 2: | CATTCCCATCCCCTAATGTG |