Insertion junction: LMJ.RY0402.192575_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.192575
Chromosome:chromosome 6
Location:3768714
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278159 CRCO,CO,CON1,CONSTANS CONSTANS-like protein involved in circadian rhythms; (1 of 1) PF00643//PF06203 - B-box zinc finger (zf-B_box) // CCT motif (CCT) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCCTCAAAGGTTCAGTCCCCAACGCTT
Internal bar code:TAGTGGTTCGTTCATTGATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:978
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1184
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACCGACCTATCGAACG
Suggested primer 2:TCGAACTCGAGCTTGTCCTT