| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192598 |
| Chromosome: | chromosome 11 |
| Location: | 1125026 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467680 | (1 of 13) 3.1.13.4 - Poly(A)-specific ribonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCCCAAACTAAACCCAAACCAACACC |
| Internal bar code: | CGGGGCAGCAAGCGGTTGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 97.0356 |
| LEAP-Seq n confirming: | 982 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTTTTGAGTGTGGTTGG |
| Suggested primer 2: | GGGGTAAGATCTGTCGGTCA |