Insertion junction: LMJ.RY0402.192610_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.192610
Chromosome:chromosome 3
Location:4297513
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174672 (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACTGCAGCACCTAAACCTTCCGTGCATC
Internal bar code:GAGTCTGGTCGCGCGTTAGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:99.2147
LEAP-Seq n confirming:3411
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTCTTCGTTTAGCCACCC
Suggested primer 2:CACACTTTCACTCACCCCCT