Insertion junction: LMJ.RY0402.192642_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.192642
Chromosome:chromosome 14
Location:2547068
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g625600 FHL10 (1 of 2) PF02010 - REJ domain (REJ) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCATGTTTATTTATGTGCGTGCTTCCT
Internal bar code:CCTTGCTCGTGCTTATCCCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:279
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1646
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCCAGTGACTCGCCTTC
Suggested primer 2:GGAATAGTCGTGGGTGCAGT