| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192653 |
| Chromosome: | chromosome 13 |
| Location: | 1373114 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571750 | (1 of 1) PTHR10050 - DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTGCGTGACGCTGCAAGCTCTGACCTCC |
| Internal bar code: | CGGGGTGATCGTTAAATTCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 99.2434 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTAACTGCAGGCCCATTG |
| Suggested primer 2: | ATCCGTTTAGCAGGTTGGTG |