| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192658 |
| Chromosome: | chromosome 10 |
| Location: | 6309445 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465300 | (1 of 89) 3.6.4.13 - RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGAGCCCTCAATGAATTGCAGTCCACT |
| Internal bar code: | CCGGTGAACGGTGCCTGTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 77.3199 |
| LEAP-Seq n confirming: | 2683 |
| LEAP-Seq n nonconfirming: | 787 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCTATGAGGTCCGTTGG |
| Suggested primer 2: | ACTTCAACAGATTGGACGGG |