| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192675 |
| Chromosome: | chromosome 3 |
| Location: | 1741986 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153750 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTGTGCTGGTGCGTGTGAAGGCGCACC |
| Internal bar code: | GGGGTAATAGATAATTGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1131 |
| LEAP-Seq percent confirming: | 99.9023 |
| LEAP-Seq n confirming: | 2046 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACGGCTGAACTGAGACC |
| Suggested primer 2: | ATAGTCAGCGAGGCAGGTGT |