| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192689 |
| Chromosome: | chromosome 9 |
| Location: | 2073563 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393950 | FHL2,FTSHI3,FTSHi3,FHL8 | (1 of 6) K03798 - cell division protease FtsH [EC:3.4.24.-] (ftsH, hflB); Putative chloroplast-import FtsH-like ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTGTCTGTGAGTGGATGCGTGAATGTG |
| Internal bar code: | ACCGGTATAAAGAGGAGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.5833 |
| LEAP-Seq n confirming: | 1434 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTAGGGCATAGGAGGGC |
| Suggested primer 2: | GGCGTTTGGTGTAGGAAAAA |