| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.192704 |
| Chromosome: | chromosome 6 |
| Location: | 5870704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288000 | (1 of 21) IPR004827 - Basic-leucine zipper domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGTTGCGGGCACAGCCGCGGCGGCAGTG |
| Internal bar code: | CTGGGTGGTGTTACGGTAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 119 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCTACCTCACCTCCTT |
| Suggested primer 2: | TCATCAGTCCACGCTACGTC |