| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192746 |
| Chromosome: | chromosome 16 |
| Location: | 4890491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685901 | (1 of 1) K18423 - exportin-2 (importin alpha re-exporter) (CSE1, CAS, XPO2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGGTAAGGAATGAGCAGCGGCCGGTG |
| Internal bar code: | GGGGGCTAGGTCGAGCCAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 99.4437 |
| LEAP-Seq n confirming: | 715 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTACCATTTCCTTGGGCA |
| Suggested primer 2: | TGAAAGGCTGCGATACAGTG |