| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192765 |
| Chromosome: | chromosome 3 |
| Location: | 3019241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163900 | PWR3 | (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAGTCGAGCGTATCCCTTTCCCCACGC |
| Internal bar code: | AGGTGTGGGGTCGCAAAATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 562 |
| LEAP-Seq percent confirming: | 96.8586 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTTGTTTTTGATGTGTG |
| Suggested primer 2: | GCACACGTTTCATTTTGTGG |