| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192835 |
| Chromosome: | chromosome 3 |
| Location: | 5180941 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182400 | (1 of 7) K18932 - palmitoyltransferase (ZDHHC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGATCCAAACGTGGTGGGCAGGGGAAGG |
| Internal bar code: | TGGCATTAGTTGCGCGACAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 70.7047 |
| LEAP-Seq n confirming: | 2428 |
| LEAP-Seq n nonconfirming: | 1006 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCTATGCTGGCTGACA |
| Suggested primer 2: | TTGCATGAACAGACACAGCA |