| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192875 |
| Chromosome: | chromosome 9 |
| Location: | 1843019 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395800 | (1 of 1) PF05907 - Eukaryotic protein of unknown function (DUF866) (DUF866) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACGGAGGCCGCCTGACGCGCTTCTGCC |
| Internal bar code: | GCAGTGACACAGTCGCAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.3902 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCGGGTTAGTTTGGGCT |
| Suggested primer 2: | CAGTGTGCACCCAGACAAAC |