Insertion junction: LMJ.RY0402.192877_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.192877
Chromosome:chromosome 3
Location:8957401
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g208833 (1 of 1) K10896 - fanconi anemia group M protein (FANCM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGAGGAGCGGCGGGAGGAGGAGGCGAAG
Internal bar code:CGTGTACACAGTCAACAGCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:85
LEAP-Seq percent confirming:3.49538
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:2402
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACCACACCAACATCAAG
Suggested primer 2:TACTGAACAGGCTGAAGCGA