| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.192894 |
| Chromosome: | chromosome 8 |
| Location: | 2309148 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370401 | CYCB1,DIV80 | (1 of 1) K05868 - cyclin B (CCNB); B-type cyclin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGCCAGCAGCAGCGGCAGGCTGCTGG |
| Internal bar code: | TTATGATGACCTTAAAATTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 79.7872 |
| LEAP-Seq n confirming: | 225 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCCGGTAAGCGGTTCT |
| Suggested primer 2: | GCATCAGCTGCAATACGAAA |