| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192902 |
| Chromosome: | chromosome 9 |
| Location: | 2259396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392652 | (1 of 1) 1.5.1.22 - Strombine dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGCACAATATACGGCACAATCATCAGG |
| Internal bar code: | TCCCTCGGTGCAGTTATATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1200 |
| LEAP-Seq percent confirming: | 99.422 |
| LEAP-Seq n confirming: | 2580 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATTGCCTGTGTTTGGCT |
| Suggested primer 2: | CTGATCAGTAGGGCTTTCGC |