Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.192912 |
Chromosome: | chromosome 8 |
Location: | 1000281 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGGGACCTGCTGACAAGCACAAGCACA |
Internal bar code: | GCGGATGCCGCTCGCCCTGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 437 |
LEAP-Seq percent confirming: | 99.58 |
LEAP-Seq n confirming: | 2134 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGCACCAACAGCTACACTA |
Suggested primer 2: | GACTTTGGGTATCAGGCCAA |