Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.192934 |
Chromosome: | chromosome 6 |
Location: | 2615727 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g269700 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCACACGTCCGAAGACATGTCGTGTCA |
Internal bar code: | CAGCCGGCCAGGCACGCTGTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 627 |
LEAP-Seq percent confirming: | 99.708 |
LEAP-Seq n confirming: | 2732 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGTTGGTGGTTGATGTGGT |
Suggested primer 2: | AACAGTCAACACCCGAAAGG |