| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.192968 |
| Chromosome: | chromosome 10 |
| Location: | 5102270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456150 | HLD3 | (1 of 1) PTHR10992:SF701 - SERINE HYDROLASE-RELATED; Haloalkane dehalogenase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCATTTGCATATGGCTCTCTCCCCAT |
| Internal bar code: | GGTTAATACCCGCGCATTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.4562 |
| LEAP-Seq n confirming: | 1463 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAGCTACTCTACGCGCT |
| Suggested primer 2: | GTGTTGTCCAGATGTGTCGG |