| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.192989 |
| Chromosome: | chromosome_7 |
| Location: | 5515166 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g351050 | VPS23 | Subunit of the ESCRT-I complex | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCGTAAGGATCACGCAGGCCAAACGTGGT |
| Internal bar code: | GCCTGTGAGGCAGGCACGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.435 |
| LEAP-Seq n confirming: | 3696 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTCGTACCCATGTCCT |
| Suggested primer 2: | AGCATGAACACCACACCAAA |