Insertion junction: LMJ.RY0402.193009_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.193009
Chromosome:chromosome 6
Location:3535299
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278110 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATTATGCGGCCGCACGTGTGCAAGCACG
Internal bar code:CAATGAGCTAACTATTGTTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:992
LEAP-Seq percent confirming:99.4541
LEAP-Seq n confirming:1822
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTCCGCAACTTTAGATCC
Suggested primer 2:CGTAGCTAGCTGCTGTGTGC