| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193023 |
| Chromosome: | chromosome 2 |
| Location: | 7577334 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143801 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCACCACTGGGGTGCTCTCTTGAGTT |
| Internal bar code: | GAGGCCGTGAGGTTGGCAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.1946 |
| LEAP-Seq n confirming: | 739 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGAAGCATGTGTACCG |
| Suggested primer 2: | ACGGATGTGGTTCAGAAAGG |