| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193091 |
| Chromosome: | chromosome 9 |
| Location: | 2124750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393550 | (1 of 1) 2.7.11.25//2.7.12.2 - Mitogen-activated protein kinase kinase kinase / MLTK // Mitogen-activated protein kinase kinase / MKK | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATCTTCGCCTCAGGACATCCACCGTCG |
| Internal bar code: | AATATGGCCGGGTCATCAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 788 |
| LEAP-Seq percent confirming: | 99.8267 |
| LEAP-Seq n confirming: | 576 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTAGCTCCCAGACCAG |
| Suggested primer 2: | GTATGTTAGGGCTGCTGGGA |