| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193124 |
| Chromosome: | chromosome 2 |
| Location: | 5049300 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104650 | (1 of 1) K14573 - nucleolar protein 4 (NOP4, RBM28) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGCGACAACCATCTCCACATCCAGCC |
| Internal bar code: | CAGGTACTCGGCGGAGGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 98.1609 |
| LEAP-Seq n confirming: | 854 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTGTCGATTATGCCGT |
| Suggested primer 2: | ACCGCGTAAAGTAAGCGAGA |