| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193133 |
| Chromosome: | chromosome 7 |
| Location: | 5541866 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351400 | MCM10 | (1 of 1) PTHR13454//PTHR13454:SF11 - FAMILY NOT NAMED // PROTEIN MCM10 HOMOLOG; Minichromosome maintenance protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCGCCACCCGCGCCCGCGCCTGCCTC |
| Internal bar code: | GAAACGTTTTTGAGCTTTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCGATATCCTGATTGTG |
| Suggested primer 2: | ACGCACACACACACACACAC |