| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193145 |
| Chromosome: | chromosome 8 |
| Location: | 4053711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACTGGAAAGATGTCGATAATCTGTCTG |
| Internal bar code: | TCGTTGGTACTGGGGTAGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 94.3522 |
| LEAP-Seq n confirming: | 1136 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCTGTTGTTGTTGATAA |
| Suggested primer 2: | GAGACATCTCCAGCAGAGGC |