| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193177 |
| Chromosome: | chromosome 2 |
| Location: | 5608335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109126 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGTTCTCGTCACGGTCCTGCGGGTGCG |
| Internal bar code: | CGCTCCTAGGCGGGGCTGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.7778 |
| LEAP-Seq n confirming: | 898 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCGGCTACAAGTACAGC |
| Suggested primer 2: | ACGGTCCATGATATCCCAAA |