Insertion junction: LMJ.RY0402.193177_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.193177
Chromosome:chromosome 2
Location:5608337
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g109126 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCTCAACATGCGACTGCTGTTGATCTT
Internal bar code:CGTCGCGCGTCGGGTAGACCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:418
LEAP-Seq percent confirming:99.7257
LEAP-Seq n confirming:6180
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCGGCTACAAGTACAGC
Suggested primer 2:ACGGTCCATGATATCCCAAA