Insertion junction: LMJ.RY0402.193193_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.193193
Chromosome:chromosome_11
Location:2739208
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g477100 FAP97 Flagellar Associated Protein, serine endopeptidase antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TCCAATTGGTAGGTGTCCGGAAGCATCTCC
Internal bar code:CGCCCCCGCTTAAGACAACGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:139
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGTAGGGATGGGGATTA
Suggested primer 2:ATCCCATTAGGAAACAGGGG