Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.193193 |
Chromosome: | chromosome_11 |
Location: | 2739208 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g477100 | FAP97 | Flagellar Associated Protein, serine endopeptidase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCAATTGGTAGGTGTCCGGAAGCATCTCC |
Internal bar code: | CGCCCCCGCTTAAGACAACGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 139 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 59 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGAGTAGGGATGGGGATTA |
Suggested primer 2: | ATCCCATTAGGAAACAGGGG |