| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193222 |
| Chromosome: | chromosome 10 |
| Location: | 896697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424200 | POLE2 | (1 of 1) K02325 - DNA polymerase epsilon subunit 2 (POLE2); DNA polymerase epsilon subunit 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTGATGATGTGGAGGCGGCGGCGGAGG |
| Internal bar code: | CTAGTCGGTGTCAGGTCCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.8653 |
| LEAP-Seq n confirming: | 1483 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTTGGTCGTGGTGAAG |
| Suggested primer 2: | GGAGCAGGTGGAGCTGATAG |