| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193273 |
| Chromosome: | chromosome 10 |
| Location: | 3408420 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444400 | (1 of 2) K16458 - centrosomal protein CEP104 (CEP104) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTAAGGCGGCACATTCGCTCTTTGCAGT |
| Internal bar code: | TGACGGCGTCGTGTGAGATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 99.1863 |
| LEAP-Seq n confirming: | 1219 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGGCATGTGTGTGTTTT |
| Suggested primer 2: | ATGGACGGGTGCAAGTCTAC |