| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193295 |
| Chromosome: | chromosome 1 |
| Location: | 3598985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCAATTCTAACCCCGACGCCACAAACCA |
| Internal bar code: | CTCTCACGCATGTGCGCCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 94.7177 |
| LEAP-Seq n confirming: | 520 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGGTGTATGTACGGGATT |
| Suggested primer 2: | TTCTCATGGTGGATCCAGGT |