| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193322 |
| Chromosome: | chromosome 16 |
| Location: | 7006889 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677765 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTAGCGTTTCTAAGATTAATTGGCGTGA |
| Internal bar code: | GCGGGCGTCTCAGCTCGGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 99.5514 |
| LEAP-Seq n confirming: | 2219 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCTGGGGCTATGTCTA |
| Suggested primer 2: | AAAGACCAGGTTTGACCGTG |