| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193332 |
| Chromosome: | chromosome_17 |
| Location: | 6208459 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g741950 | BBS1 | Bardet-Biedl syndrome-1 associated protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTTGTTGGGGTTTGTAAGGTCAACTTCGG |
| Internal bar code: | TTGGGGCATTTGAGGCAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 98.7509 |
| LEAP-Seq n confirming: | 2767 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGGTGATCGTTGTCAC |
| Suggested primer 2: | GTCGTTTTGTCCCACTCGAT |