Insertion junction: LMJ.RY0402.193332_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.193332
Chromosome:chromosome 17
Location:6208459
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g741950 BBS1 Bardet-Biedl syndrome-1 associated protein; (1 of 1) K16746 - Bardet-Biedl syndrome 1 protein (BBS1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGTTGGGGTTTGTAAGGTCAACTTCGG
Internal bar code:TTGGGGCATTTGAGGCAAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:921
LEAP-Seq percent confirming:98.7509
LEAP-Seq n confirming:2767
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAGGTGATCGTTGTCAC
Suggested primer 2:GTCGTTTTGTCCCACTCGAT