| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193334 |
| Chromosome: | chromosome 7 |
| Location: | 6083489 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355433 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCTTTACGAGCAACACATTGGCACATT |
| Internal bar code: | TGAGCGCATCGCTGGCAGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.7605 |
| LEAP-Seq n confirming: | 2499 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCACCTCAGATTGTGCT |
| Suggested primer 2: | TGCGCGCTAAACACTTATTG |