Insertion junction: LMJ.RY0402.193336_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.193336
Chromosome:chromosome 4
Location:1271602
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g215001 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCCCGACAATCTTGGACGCGAAGACCAT
Internal bar code:AGCAGGCGGAGATTTAGCGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:450
LEAP-Seq percent confirming:95.6487
LEAP-Seq n confirming:3605
LEAP-Seq n nonconfirming:164
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGTCCATGTCACGTCCT
Suggested primer 2:AGGTGGATGGAGAAATGTGC