| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193350 |
| Chromosome: | chromosome 12 |
| Location: | 7171254 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558800 | (1 of 2) IPR000104//IPR017923 - Antifreeze protein, type I // Transcription factor IIS, N-terminal | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGCATGTGCAACACCAGGGGTTCTGG |
| Internal bar code: | CGACTCGACTGATCTCTGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.7308 |
| LEAP-Seq n confirming: | 5557 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGCATTACATGCACCA |
| Suggested primer 2: | ATGTCGCAATCTCTCTGGCT |