| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193364 |
| Chromosome: | chromosome 3 |
| Location: | 5500958 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGACAGGGCACGCACGTCTCCTCCGTC |
| Internal bar code: | ACTTGTGGGGGACGGACCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 507 |
| LEAP-Seq percent confirming: | 99.5759 |
| LEAP-Seq n confirming: | 5400 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGTTATGGCCTTACGGG |
| Suggested primer 2: | TACTGCCCCAACCCTTACAG |