| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193369 |
| Chromosome: | chromosome 16 |
| Location: | 2375948 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660000 | CMT1A,CPLD63 | (1 of 2) PTHR12608:SF7 - GDT1-LIKE PROTEIN 2, CHLOROPLASTIC; Mn transport into chloroplast | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCAGTCGCTGTGCGTTGGCGTGGCAT |
| Internal bar code: | GCGCTGACGGGGGAGCGGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 99.7084 |
| LEAP-Seq n confirming: | 2735 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAAGCTGGAGCTGTGCGT |
| Suggested primer 2: | GGCGGTGTTGTAGATCTGGT |