| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193375 |
| Chromosome: | chromosome 17 |
| Location: | 1329524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGAATGGGAACATGGGCGGGCGTGGC |
| Internal bar code: | GAATACGTAGCCGCGGTGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 62.963 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGTATTCCCCTCCTGC |
| Suggested primer 2: | GACGTCGCTACAGAAGGAGG |