Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.193386 |
Chromosome: | chromosome 14 |
Location: | 2040536 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g621800 | GT90-27,GT90F27 | GT90 family protein 27; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGAAGGGCCTTTTCCCGGCGTTACAGG |
Internal bar code: | GGTGCGCGACTGCACGTTCCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 804 |
LEAP-Seq percent confirming: | 99.6109 |
LEAP-Seq n confirming: | 768 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCGTATGTGTGGAAGCCA |
Suggested primer 2: | GTCAAAATGTCAGCTTGCGA |