Insertion junction: LMJ.RY0402.193392_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.193392
Chromosome:chromosome_13
Location:3741804
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g589450 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGCACTCCACGTCGCTCACATAGCTCAGGG
Internal bar code:GTATATGTAAACGTGACGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:264
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:368
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAACGAAATTGTGAGCCC
Suggested primer 2:GAGTGGCGCTACAGTGACAA