Insertion junction: LMJ.RY0402.193393_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.193393
Chromosome:chromosome_9
Location:5020043
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g398104 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TCCAGACGTTGAATGCTTGGTTGGCAGGCC
Internal bar code:TATTCGTAAATTGTATGGCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:279
LEAP-Seq percent confirming:99.5894
LEAP-Seq n confirming:8489
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGGAAGGTAACAAGAGAG
Suggested primer 2:CTCTGTGACCCTCATCAGCA