Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.193393 |
Chromosome: | chromosome_9 |
Location: | 5020043 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g398104 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCAGACGTTGAATGCTTGGTTGGCAGGCC |
Internal bar code: | TATTCGTAAATTGTATGGCTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 279 |
LEAP-Seq percent confirming: | 99.5894 |
LEAP-Seq n confirming: | 8489 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCGGAAGGTAACAAGAGAG |
Suggested primer 2: | CTCTGTGACCCTCATCAGCA |