| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193420 |
| Chromosome: | chromosome 10 |
| Location: | 4224588 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450100 | (1 of 1) K04957 - hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGCCAAACACACACGCACACTTGTGC |
| Internal bar code: | GCGTTACTGCCGACGCGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.4541 |
| LEAP-Seq n confirming: | 1822 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACACCGT |
| Suggested primer 2: | ACACAGATCAGGATGGGGAG |