Insertion junction: LMJ.RY0402.193424_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.193424
Chromosome:chromosome 12
Location:774823
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g495600 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATTGCACTGCGGCGAGTTGGGCCCCGA
Internal bar code:GCGTCACTTGCGCAAACCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:99.7623
LEAP-Seq n confirming:7136
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCGACTTCGCCATCTAC
Suggested primer 2:CTAATAAGGGGCACACCGAA